HPLC fingerprint of ethyl acetate extraction of Saxifraga stolonifera / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1026-1029, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-350667
ABSTRACT
<p><b>OBJECTIVE</b>To establish an HPLC fingerprint of ethyl acetate extraction of Saxifraga stolonifera.</p><p><b>METHOD</b>The HPLC analysis was performed on a Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column with isocratic elution of acetonitrile-0.05% phosphoric acid at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 256 nm and the column temperature was set at 30 degrees C.</p><p><b>RESULT</b>The HPLC fingerprint of ethyl acetate extraction of S. stolonifera has been established. There were fifteen common peaks, seven of which were identified by reference substances. The RSD of relative retention time was less than 3% in the precision and repeated tests. Eleven samples from different area can be distinguished from their fingerprints.</p><p><b>CONCLUSION</b>This method is reasonable and reliable and can be used for quality control of S. stolonifera.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Quality Control
/
Reference Standards
/
Drugs, Chinese Herbal
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Saxifragaceae
/
Acetates
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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