Research of DNA barcode of Teseudinis Carapax et Planstrum and its adulterants based on COI gene sequence / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 947-950, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-350684
ABSTRACT
<p><b>OBJECTIVE</b>To use COI gene on the Mauremys reevesii and its adulterants by molecular identification. Search a rapid, accurate method of identification of Teseudinis Carapax et Planstrum and its adulterants.</p><p><b>METHOD</b>We collected 8 species of the authentic and adulterants of teseudinis carapax et planstrum in a nationwide then, extracted DNA, got the COI sequences. Use ContigExpress, Dnaman, Edit Sequence and Mega 5 to analyze the variable site and construct the N-J tree.</p><p><b>RESULT</b>Compare with the authentic Teseudinis Carapax et Planstrum, the adulterant exist lots of variable site. The N-J tree Indicates that the same genus belong together and each species belong to relatively independent branch.</p><p><b>CONCLUSION</b>Based on the COI gene, the technology of DNA bar code can be a excellent identification of Teseudinis Carapax et Planstrum and its adulterants.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Quality Control
/
Reference Standards
/
Turtles
/
Molecular Sequence Data
/
Base Sequence
/
Classification
/
Sequence Analysis, DNA
/
Electron Transport Complex IV
/
Reptilian Proteins
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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