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Research of DNA barcode of Teseudinis Carapax et Planstrum and its adulterants based on COI gene sequence / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-350684
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To use COI gene on the Mauremys reevesii and its adulterants by molecular identification. Search a rapid, accurate method of identification of Teseudinis Carapax et Planstrum and its adulterants.</p><p><b>METHOD</b>We collected 8 species of the authentic and adulterants of teseudinis carapax et planstrum in a nationwide then, extracted DNA, got the COI sequences. Use ContigExpress, Dnaman, Edit Sequence and Mega 5 to analyze the variable site and construct the N-J tree.</p><p><b>RESULT</b>Compare with the authentic Teseudinis Carapax et Planstrum, the adulterant exist lots of variable site. The N-J tree Indicates that the same genus belong together and each species belong to relatively independent branch.</p><p><b>CONCLUSION</b>Based on the COI gene, the technology of DNA bar code can be a excellent identification of Teseudinis Carapax et Planstrum and its adulterants.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Quality Control / Reference Standards / Turtles / Molecular Sequence Data / Base Sequence / Classification / Sequence Analysis, DNA / Electron Transport Complex IV / Reptilian Proteins Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2013 Type: Article
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Quality Control / Reference Standards / Turtles / Molecular Sequence Data / Base Sequence / Classification / Sequence Analysis, DNA / Electron Transport Complex IV / Reptilian Proteins Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2013 Type: Article