Peptides analysis in digested edible bird's nest by HPLC-MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 714-719, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-350699
ABSTRACT
Edible bird's nest contains lots of glycoproteins. The glycosylation inhomogeneity for glycoprotein often results in wide range of molecular weight and the difficulty for protein separation and charaterization. In this paper, proteins in the edible bird's nest were extracted using multiple extractions, and then digested by PNgase F and trypsin. The digest mixture was separated with HPLC, and peptides were identified based on MS/MS data searching. The results indicated that the extracted proteins were amount to 79.7% of total protein in the edible bird's nest. More than 20 species of peptides in the digested mixture were identified. The sequences of these peptides showed similarity with some proteins from Swiss-prot. The research indicated that deglycosylation, tryptic digestion coupled with HPLC-MS/MS is a proper strategy for characterization of proteins in the edible bird's nest.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptide Fragments
/
Mass Spectrometry
/
Birds
/
Glycoproteins
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Amino Acid Sequence
/
Proteolysis
/
Medicine, Chinese Traditional
/
Metabolism
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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