Determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets by HPLC isocratic elution / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 691-693, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-350704
ABSTRACT
<p><b>OBJECTIVE</b>To establish an HPLC method for determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets.</p><p><b>METHOD</b>HPLC isocratic elution was adopted, with the C18 column (4.6 mm x 250 mm, 5 microm) as the chromatographic column, methanol-water-phosphoric acid (45 55 0.2) as the mobile phase. The flow rate was set at 1.0 mL x min(-1), and column temperature was set at 30 degrees C. The detection wavelength was 328 nm, and the sample size was 20 microL.</p><p><b>RESULT</b>At concentrations ranging between 2.200-44.00, 24.20-242.0 mg x L(-1), peak area of chlorogenic acid and baicalin showed good linear relationship, the average recoveries were 99.63% (RSD 1.8%, n = 9) and 99.72% (RSD 1.9%, n = 9), respectively.</p><p><b>CONCLUSION</b>The method is simple that it can accurately measure the content of chlorogenic acid and baicalin in Yinhuang film-coated tablets.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tablets
/
Flavonoids
/
Drugs, Chinese Herbal
/
Linear Models
/
Chemistry
/
Chlorogenic Acid
/
Chromatography, High Pressure Liquid
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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