Pre-freezing equilibration improves post-thaw human semen parameters / 中华男科学杂志
National Journal of Andrology
;
(12): 886-889, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-350818
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of pre-freezing equilibration on the cryo-survival of human sperm and to optimize the protocol of direct fumigation for the freeze-thawing of human sperm.</p><p><b>METHODS</b>We collected 50 semen samples from healthy donors, each subjected to cryopreservation with 3 different methods:
non-equilibration freezing (Group A), 10-min equilibration at room temperature before freezing (Group B), and 10-min equilibration at 4 degrees C before freezing (Group C). We examined all the post-thaw semen samples by computer-assisted semen analysis for the sperm motility parameters, and detected the sperm vitality and deformity index (SDI).</p><p><b>RESULTS</b>The recovery rate of progressive sperm motility was (61.88 +/- 16.94)% in Group C, remarkably higher than in A ([48.61 +/- 16.44]%) and B ([49.41 +/- 13.77]%) (P < 0.05), but with no significant difference between the latter two. And there were no significant differences in sperm vitality and SDI among the three groups.</p><p><b>CONCLUSION</b>Ten-minute equilibration at 4 degrees C before freezing can evidently improve the progressive motility of sperm in addition to its advantages of easy operation and controllable experimental condition.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Semen Preservation
/
Sperm Count
/
Sperm Motility
/
Sperm Banks
/
Cryopreservation
/
Semen Analysis
/
Methods
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2013
Type:
Article
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