ICSI causes abnormal H3K9 methylation in the male pronuclei and growth retardation of mouse embryos / 中华男科学杂志
National Journal of Andrology
;
(12): 593-598, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-350854
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the safety of intracytoplasmic sperm injection (ICSI) in the mouse model.</p><p><b>METHODS</b>We simulated clinical ICSI technology and comprehensively evaluated it by parthenogenetic activation, immunofluorescence, embryo transplantation, examination of early implantation, and measurement of the crown-rump length (CRL).</p><p><b>RESULTS</b>ICSI significantly reduced the ability of preimplantation embryo development of the mouse, especially after the 8-cell stage (P < 0.01). The fluorescence of H3K9 dimethylation was abnormal at the male pronuclei of the embryos derived from ICSI. Further examination of the development of the transferred ICSI embryos indicated no significant difference in the rate of early implantation at E5. 5 days as compared with normal fertilization (P = 0.6), but the percentage of "normal embryos" was decreased significantly at E9.5 days (P < 0.01). Obvious growth retardation phenotype was observed even in the normal ICSI embryos at E9.5 days.</p><p><b>CONCLUSION</b>ICSI might result in growth retardation of embryos by affecting H3K9 dimethylation in the male pronuclei.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Histones
/
Sperm Injections, Intracytoplasmic
/
Embryonic Development
/
Jumonji Domain-Containing Histone Demethylases
/
Metabolism
/
Methylation
/
Mice, Inbred ICR
Type of study:
Etiology study
Limits:
Animals
/
Pregnancy
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2013
Type:
Article
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