Determination of acetylharpagide in Ajuga decumbens by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 289-290, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-350958
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens.</p><p><b>METHOD</b>The chromatographic conditions were as follows a Phenomenex Luna C18 column was used, the mobile phase was composed of acetontrile-water (1585), the flow rate was 1.0 mL x min(-1) and the UV absorbance detection was set at 197 nm.</p><p><b>RESULT</b>Linearity of acetylharpagide was in the range of 0.6-3.6 microg (r = 0.9993), and the average recovery and RSD were 99.13% and 2.48%, respectively.</p><p><b>CONCLUSION</b>The contents of acetylharpagide ranged 0.40%-6.39% in A. decumbens. The method was simple, accurate and sensitive.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Pyrans
/
Drugs, Chinese Herbal
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Ajuga
/
Methods
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
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