The effect of genetic polymorphism of the exon 2 of the beta-lactoglobulin gene on the milk composition in Chinese Holstein / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 333-337, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-351161
ABSTRACT
<p><b>OBJECTIVE</b>In order to study the effect of the polymorphism at the exon2 region of the (3-LG allele gene on milk composition and yield.</p><p><b>METHODS</b>The single-strand conformation polymorphism method (PCR-SSCP) was used to analyze for polymorphism the exon2 region of the 3-LG gene (NCBI accession number DQ489319) in Chinese Holstein.</p><p><b>RESULTS</b>Eight SSCP patterns were detected in the fragments ab, abc, abd, abe, abcd, abce, abde and abcde, and the patterns frequencies as follows 0.14, 0.10, 0.27, 0.23, 0.05, 0.04, 0.11 and 0.06 (P < 0.05); Six single nucleotide polymorphism (SNP) were detected in this study sitel C>T, site2 T>C, site3 C>T, site4 C>C, site5 C> A, site6 A>T or C, and the polymorphism infonnation content (PIC) of these SNPs were in median or high polymorphism (PIC > 0.25).</p><p><b>CONCLUSION</b>These SNPs at the exon2 region of the beta-LG gene were remarkably and affected milk performance traits (milk yield, protein and fat contents) in Chinese Holstein.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
Chemistry
/
China
/
Polymerase Chain Reaction
/
Exons
/
Classification
/
Polymorphism, Single-Stranded Conformational
/
Milk
/
Alleles
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2011
Type:
Article
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