The relationship between endoplasmic reticulum stress and its particular apoptosis way caspase-12 and apoptosis in renal cortex of diabetic rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 236-240, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-351189
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expressions of 78-kDa glucose-regulated protein (GRP78) and Caspase-12 and their relationship with apoptosis in renal cortex of diabetic rats.</p><p><b>METHODS</b>Uninephrectomized Wistar rats were used to induce diabetes by intraperitoneal injection of Streptozotocin (STZ 65 mg/kg). After 8 weeks, the expression and distribution of GRP78, Caspase-12, proliferating cell nuclear antigen (PCNA) were examined by immunohistochemistry. Flow cytometry was used to detect the levels of protein of GRP78 and Caspase-12. Apoptosis was evaluated by means of terminal deoxynucleotidyl transferase-mediated d-UDP nick-end labeling (TUNEL) and Flow cytometry. Serum creatinine, blood urea nitrogen and 24-hour urine protein excretion were checked.</p><p><b>RESULTS</b>Compared with those in normal control group, the numbers of apoptosis and the expression of GRP78, Caspase-12 in glomerular and tubular cells were much higher in the diabetic kidneys at 8 weeks. There was no significant difference between group A and group B.</p><p><b>CONCLUSION</b>Activation of endoplasmic reticulum stress may play an important role in the development of diabetic nephropathy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Rats, Wistar
/
Apoptosis
/
Proliferating Cell Nuclear Antigen
/
Diabetes Mellitus, Experimental
/
Diabetic Nephropathies
/
Caspase 12
/
Endoplasmic Reticulum Stress
/
Heat-Shock Proteins
/
Kidney Cortex
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2011
Type:
Article
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