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Expression and function of autophagy after ischemia/reperfusion in rats hippocampus neuron / 中国应用生理学杂志
Chinese Journal of Applied Physiology ; (6): 187-191, 2011.
Article in Chinese | WPRIM | ID: wpr-351203
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of autophagy after ischemia/reperfusion and its possible function in rats hippocampus neurons.</p><p><b>METHODS</b>After 2 hours oxygen-glucose deprivation and different periods time of reperfusion (OGD/R) treatment in primary hippocampal neurons, neuron viability was evaluated by MTT assay, specific structure of autophagosome and specific protein of autophagy microtubule-associated protein 1 light chain 3 B (LC3B) were detected by transmission electron microscope and immunofluorescence respectively. The inhibitor of autophagy 3-Methyladenine (3-MA) was also used to exam the viability of neurons.</p><p><b>RESULTS</b>Treatment by OGD/R markedly reduced neuronal viability. Compared to the control group, autophagy existed in different time periods after OGD/R shown both in transmission electron microscope and immunofluorescence. Application of 3-MA significantly reduced neuronal viability.</p><p><b>CONCLUSION</b>Oxygen-glucose deprivation can activate autophagy in rat hippocampus neurons, which may resist the injury during ischemia/reperfusion.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Physiology / Autophagy / Reperfusion Injury / Cell Hypoxia / Brain Ischemia / Culture Media, Serum-Free / Rats, Sprague-Dawley / Cell Biology / Primary Cell Culture Limits: Animals Language: Chinese Journal: Chinese Journal of Applied Physiology Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Physiology / Autophagy / Reperfusion Injury / Cell Hypoxia / Brain Ischemia / Culture Media, Serum-Free / Rats, Sprague-Dawley / Cell Biology / Primary Cell Culture Limits: Animals Language: Chinese Journal: Chinese Journal of Applied Physiology Year: 2011 Type: Article