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Research on quality standards of Euonymus fortunei / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-351246
Responsible library: WPRO
ABSTRACT
To establish quality standards of Euonymus fortunei, and supply scientific evidence for the quality control of Euonymus fortunei. Empirical and microscopic identification methods were adopted to observe morphological and histological characters. The contents of water, total ash, acid-insoluble ash and alcohol-soluble extractive were analysed according to the methods of Chinese Pharmaco- poeia (2010). Dulcitol and reference herbs were used to identify materia medica of Euonymus fortunei by TLC method. The total flavonol glycosides contents were analysed by HPLC method, using quercetin and kaempferol as reference substances. Quercetin and kaempferol were separated on a C18 column (4.6 mm x 250 mm, 5 μm) with methanol-0.1% formic acid(5149) as the mobile phase and detected at 366 nm. The flavonoid aglycones content was then multiplied by a conversion coefficient, and the result was the total flavonol glycosides content. The macroscopical identification, microscopic features and TLC methods were proper. The average contents of water, total ash, acid-insoluble ash, alcohol-soluble extractive and total flavonol glycosides were 8.76%, 6.48%, 0.31%, 17.48% and 0.211% , respectively. The quality standards established on the basis of the research results were suitable for the quality evaluation of Euonymus fortunei.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Quality Control / Reference Standards / Mass Spectrometry / Drugs, Chinese Herbal / Chemistry / China / Chromatography, High Pressure Liquid / Chromatography, Thin Layer / Euonymus Type of study: Practice guideline Country/Region as subject: Asia Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Quality Control / Reference Standards / Mass Spectrometry / Drugs, Chinese Herbal / Chemistry / China / Chromatography, High Pressure Liquid / Chromatography, Thin Layer / Euonymus Type of study: Practice guideline Country/Region as subject: Asia Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article