Your browser doesn't support javascript.
loading
Determination of phenols and triterpenoid saponins in stems of Sargentodoxa cuneata / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-351248
Responsible library: WPRO
ABSTRACT
The methods to determine the total phenols, total saponins, and marker constituents salidroside, chlorogenic acid and 3, 4-dihydroxy-phenylethyl-β-D-glucopyranoside in the samples of Sargentodoxae Caulis were established to provide the evidence for the improvement and revision of the quality standard of the crude material recorded in the Chinese Pharmacopoeia (2015 Edition). The content of total phenols was determined by ultraviolet spectrophotometry, using gallic acid as a reference substance. The content of total saponins was determined by ultraviolet spectrophotometry, using 3-O-[β-D-xylopyranosyl-(1-2)-O-β-D-glucuronopyranosyl]-28-O-[β-D-glucopyranosyl] asiatic acid as a reference substance. The contents of salidroside, chlorogenic acid and 3,4-dihydroxy-phenylethyl-β-D-glucopyranoside were detected by HPLC. The linear ranges were 1.01-7.04 mg x L(-1) for total phenols, 37.7-201 μg for total saponins, 0.025 8-1.55 μg for salidroside, 0.076 2-5.44 μg for chlorogenic acid, and 0.064 9-3.47 μg for 3,4-dihydroxy-phenylethyl-βP-D-glucopyranoside, respectively. Their average recoveries were 99.12%, 99.11% 105.5%, 99.08%, and 101.6%, respectively. The contents of total phenols and total saponins were 3. 04% -11. 9% and 0. 87% -3. 63%. The contents of salidroside, chlorogenic acid and 3,4-dihydroxy-phenylethyl-β-D-glucopyranoside fluctuated from 0.018% to 0. 572%, from 0.041% to 1.75% and from 0.035% to 1.32%. The established methods were reproducible, and they could be used for the quality control of Sargentodoxae Caulis. The present investigation suggested that total phenols, salidroside, and chlorogenic acid should be recorded in the quality standard of Sargentodoxae Caulis and their contents should not be less than 6.8% for total phenols, 0.040% for salidroside, and 0.21% for chlorogenic acid.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Saponins / Triterpenes / Drugs, Chinese Herbal / Chemistry / China / Chromatography, High Pressure Liquid / Plant Stems / Magnoliopsida / Phenol Country/Region as subject: Asia Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article
Full text: Available Index: WPRIM (Western Pacific) Main subject: Saponins / Triterpenes / Drugs, Chinese Herbal / Chemistry / China / Chromatography, High Pressure Liquid / Plant Stems / Magnoliopsida / Phenol Country/Region as subject: Asia Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article