Simultaneous determination of seven components in Pudilan Xiaoyan oral liquid by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1747-1750, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-351272
ABSTRACT
<p><b>OBJECTIVE</b>To establish an HPLC method for the content determination of baicalin, wogonin, chlorogenic acid, caffeic acid, cichoric acid, corynoline and adenosine in Pudilan Xiaoyan oral liquid.</p><p><b>METHOD</b>The analysis was performed on a Phenomenex Luna C18 column (4.6 mm x 250 mm, 5 µm) with a gradient mobile phase of methanol-0.1% trifluoroacetic acid solution system at flow rate of 1.0 mL · min(-1). The detective wavelength was at 280 nm. The column temperature was 30 °C.</p><p><b>RESULT</b>The standard curves of seven studied components show good linearity in their concentration ranges with r ≥ 0.999 6. The average recovery was 98.73%-102.1% with RSD less than 2.6%.</p><p><b>CONCLUSION</b>The method is rapid, simple and accurate, and can be applied for the quality control of Pudilan Xiaoyan oral liquid.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Succinates
/
Flavonoids
/
Caffeic Acids
/
Drugs, Chinese Herbal
/
Chlorogenic Acid
/
Chromatography, High Pressure Liquid
/
Flavanones
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Type:
Article
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