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Determination of six C-Glycoside flavones and antitumor activity of water-soluble total flavonoids from Isodon lophanthoides var. gerardianus / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 1543-1547, 2015.
Article in Chinese | WPRIM | ID: wpr-351311
ABSTRACT
This research established an HPLC method for determination of six C-Glycoside flavones of warer-soluble total flavonoids from Isodon lophanthoides var. gerardianus (Benth.) H. Hara, and studied the antitumor activity of the warer-soluble total flavonoids. The HPLC system consisted of Kromasil 100-5 C18 (4.6 mm x 250 mm, 5 microm) column and a solution system of methanol, acetonitrile and 0.5% formic acid gradient elution at a flow rate of 0. 8 mL x min(-1) and the wavelength of detector was at 334 nm. The column temperature was 25 degrees C. The antitumor activity of water-soluble flavonoids was assayed using HepG2 cell as the tested cell. The linear ranges of vicenin II, vicenin III, isoschaftoside, schaftoside, vitexin, 6, 8-di-C-a-L-arabinosylapigenin were 0.25-2.53, 0.12-1.20, 0.37-3.69, 0.16-1.63, 0.19-1.92, 0.14-1.42 microg, respectively. The average recoveries (n = 6) were 99.6% (RSD 0.87%), 100.2% (RSD 2.0%), 99.6% (RSD 1.8%), 97.9% (RSD 1.5%), 98.8% (RSD 1.2%), 98.6% (RSD 1.2%), respectively. After exposure in 24, 48, 72 h, the total flavonoids showed inhibitory effect on the proliferation of HepG2 cells with IC50 as the evaluation index, the IC50 values of 1.89, 1.71, 1.51 g x L(-1), respectively. The method is quick, simple and accurate with good re- producibility, and can be used for determination of vicenin II, vicenin III, isoschaftoside, schaftoside, vitexin, 6, 8-di-C-a-L-arabino- sylapigenin in the warer-soluble total flavonoids from L lophanthoides var. gerardianus. The warer-soluble total flavonoids from L lophanthoides have inhibitory effect on the proliferation of HepG2 cells.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Isodon / Cell Line, Tumor / Flavones / Cell Proliferation / Monosaccharides / Antineoplastic Agents, Phytogenic Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Isodon / Cell Line, Tumor / Flavones / Cell Proliferation / Monosaccharides / Antineoplastic Agents, Phytogenic Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article