Optimization of xylose fermentation for ethanol production by Candida shehatae HDYXHT-01 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 404-411, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-351519
ABSTRACT
Plackett-Burman (PB) design and central composite design (CCD) were applied to optimize of xylose fermentation for ethanol production by Candida shehatae HDYXHT-01. The PB results showed that (NH4)2SO4, KH2PO4, yeast extract and inoculum volume were the main affecting factors. With ethanol productivity as the target response, the optimal fermentation was determined by CCD and response surface analysis (RSM). The optimal fermentation conditions were (NH4)2SO4 1.73 g/L, KH2PO4 3.56 g/L, yeast extract 2.62 g/L and inoculum volume 5.66%. Other fermentation conditions were xylose 80 g/L, MgSO47H20 0.1 g/L, pH 5.0 and 250 mL flask containing 100 mL medium and cultivated at 30 degrees C for 48 h and the agitation speed was 140 r/min. Under this fermentation conditions, ethanol productivity was 26.18 g/L, which was 1.15 times of the initial.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Xylose
/
Industrial Microbiology
/
Candida
/
Ethanol
/
Fermentation
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2011
Type:
Article
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