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Isolation, cultivation and identification of adipose-derived stem cell in bovines / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1645-1651, 2010.
Article in Chinese | WPRIM | ID: wpr-351553
Responsible library: WPRO
ABSTRACT
To obtain bovine adipose-derived stem cells (ADSCs), bovine ADSCs were digested in collagenase type I solution. The growth curve of ADSCs was checked by cell counting. Chromosome analysis was checked. The molecular markers of ADSCs were detected with immunofluorescence staining. The morphology of ADSCs was identical to fibroblast like and the cells showed active proliferative ability. Vimentin, CD49d and CD13 antigens were detected, but CD34 antigen was negative. Alkaline phosphatase activity was greater in ADSCs during calcification, and Alizarin Red staining was positive. Lipid droplets were apparent around cells during adipogenesis, and Oil Red-O staining was positive. The results demonstrated that ADSCs could be used as seed cells for tissue engineering due to the simple isolation, differentiation and stable and active growth.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Cattle / Cell Differentiation / Cell Separation / Cells, Cultured / Adipose Tissue / Cell Biology / Tissue Engineering / Mesenchymal Stem Cells / Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Cattle / Cell Differentiation / Cell Separation / Cells, Cultured / Adipose Tissue / Cell Biology / Tissue Engineering / Mesenchymal Stem Cells / Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2010 Type: Article