Cyclosporin A Inhibits Albumin Synthesis in Huh7 Cells
The Korean Journal of Internal Medicine
;
: 314-319, 2011.
Article
in English
| WPRIM
| ID: wpr-35157
ABSTRACT
BACKGROUND/AIMS:
Hypoalbuminemia occurs frequently in renal transplant recipients immediately after renal transplantation. We studied the regulation of hepatic albumin synthesis by cyclosporin A (CsA) in Huh7 cells.METHODS:
Huh7 cells were incubated with various concentrations of CsA for 4, 8, 16, and 24 hours. Albumin was measured in Huh7 cell-conditioned medium by sandwich enzyme-linked immunosorbent assay and Western blot. Albumin mRNA expression was analyzed by Northern blotting in CsA-treated cells.RESULTS:
CsA (10(-7)-10(-4) M) inhibited albumin synthesis in Huh7 cells in a dose- dependent manner. A Western blot analysis for albumin in the conditioned medium released from CsA-treated (10(-7)-10(-5) M) cells also showed significant inhibition of albumin synthesis in a dose-dependent manner. Vehicle (olive oil) did not affect albumin synthesis. In contrast, a Northern blot analysis revealed no inhibition of albumin mRNA expression by CsA at any time point from 1-24 hours, indicating that the inhibition of albumin synthesis occurred at the translational level.CONCLUSIONS:
Our results suggest that inhibition of hepatic albumin synthesis by high dose CsA contributes to the hypoalbuminemia in renal transplant recipients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
RNA, Messenger
/
Serum Albumin
/
Enzyme-Linked Immunosorbent Assay
/
Gene Expression Regulation, Neoplastic
/
Cell Survival
/
Blotting, Western
/
Blotting, Northern
/
Cyclosporine
/
Culture Media, Conditioned
Limits:
Humans
Language:
English
Journal:
The Korean Journal of Internal Medicine
Year:
2011
Type:
Article
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