Mechanism of DNA transformation based on mineral nanofibers and method improvement / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1379-1384, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-351583
ABSTRACT
Sepiolite--an inexpensive, resourceful, fibrous yet inoffensive mineral--made DNA transformation rapid, simple and efficient but the mechanism for DNA transformation was still unclear. Through RNA competition test, we proposed the different transforming mechanisms from the previous report. Meanwhile, we optimized the transforming method and could transfer a colony stored at 4 degrees C for a month with plasmid through sepiolite fibers. The cells could be transformed well without competent cells preparation or incubation process. In sum, this was a novel potential transforming method, which could be explored further if the chemical method and electroporation could not be used.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transformation, Bacterial
/
DNA, Bacterial
/
Chemistry
/
Magnesium Silicates
/
Electroporation
/
Mineral Fibers
/
Nanofibers
/
Genetics
/
Methods
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2010
Type:
Article
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