Enhancement of 2-keto-L-gulonic acid production using three-stage pH control strategy / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1263-1268, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-351598
ABSTRACT
The aim of this study was to improve the 2-keto-L-gulonic acid (2-KLG) production efficiency by Ketogulonicigenium vulgare and Bacillus megaterium by using multi-stage pH control strategy. The effect of pH on the cell growths and 2-KLG production showed that the optimum pH for K. vulgare and B. megaterium cell growth were 6.0 and 8.0, respectively, while the optimum pH for 2-KLG production was 7.0. Based on the above results, we developed a three-stage pH control strategy the pH was kept at 8.0 during the first 8 h, then decreased to 6.0 for the following 12 h, and maintained at 7.0 to the end of fermentation. With this strategy, the titer, productivity of 2-KLG and L-sorbose consumption rate were achieved at 77.3 g/L, 1.38 g/(L x h) and 1.42 g/(L x h), respectively, which were 9.7%, 33.2% and 25.7% higher than the corresponding values of the single pH (pH 7.0) control model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sorbose
/
Sugar Acids
/
Bacillus megaterium
/
Chemistry
/
Culture Media
/
Rhodobacteraceae
/
Fermentation
/
Hydrogen-Ion Concentration
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2010
Type:
Article
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