P75 neurotrophin receptor mRNA sequential expression and significance after Cauda equina compression in rats / 中国骨伤
China Journal of Orthopaedics and Traumatology
;
(12): 509-513, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-351690
ABSTRACT
<p><b>OBJECTIVE</b>To observe the mRNA expression of p75NTR (p75 neurotrophin receptor) and the amount of neuronal cells apoptosis in lumbar-sacral spinal cord at different time points after the acute cauda equina compression in rats and to explore their correlation.</p><p><b>METHODS</b>Sixty adult female Sprague Dawley(SD) rats were randomly divided into the normal control group and the compression groups. The acute cauda equine compression model was established as placing a silicon gel rubber at L(3,4) level of the vertebral canal which represented about 70% to 80% compression to the cross section. The whole L(1,2) level of spinal cords were harvested at 1, 3, 5, 7, 14, 28 d after operation in compression group. Tunel method was applied to observe cell apoptosis and RT-PCR was used to detect the p75NTR mRNA expression. SPSS 13.0 statistical software was adopted to help analysis.</p><p><b>RESULTS</b>In the compression group, both the nerve cells apoptosis and the p75 mRNA expression existed the trend of low-high-low synchronally compared with the control group, there was a significant difference (P < 0.05) among comprssion groups at different time points,there was a significant difference in changes (P < 0.05). p75NTR of mRNA expression and lumbosacral nerve cells apoptosis was in a positive correlation.</p><p><b>CONCLUSION</b>After acute cauda equina compression, p75NTR mRNA expression is closely related to the neuronal apoptosis, which plays an important role in the molecular mechanism of the CES.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polyradiculopathy
/
Spinal Cord Compression
/
RNA, Messenger
/
Cauda Equina
/
Rats, Sprague-Dawley
/
Apoptosis
/
Receptor, Nerve Growth Factor
/
Disease Models, Animal
/
Genetics
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Orthopaedics and Traumatology
Year:
2011
Type:
Article
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