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Determination of papaverine in Qiangli Pipalu by HPLC / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-351752
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for the determination of the papaverine content in Qiangli Pibalu by HPLC.</p><p><b>METHOD</b>A C18 column with a solvent system of acetonitrile-0.02 mol x L(-1) sodium dihydrogen phosphate (0.2% triethylamine, phosphoric acid, at pH 3) (2575) and UV detection 240 nm were used. The flow rate was 1.0 mL x min(-1). The column temperature was maintained at 40 degrees C.</p><p><b>RESULT</b>There was a good linear relationship between the absorption value and the concentration in the range of 0.020 2-0.100 5 microg for papaverine. The average recovery rates were 99.1% (RSD 2.3%).</p><p><b>CONCLUSION</b>The method is simple, accurate and can be used to determine the contents in Qiangli Pibalu.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Antitussive Agents / Papaver / Papaverine / Pharmacology / Plants, Medicinal / Quality Control / Drugs, Chinese Herbal / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Antitussive Agents / Papaver / Papaverine / Pharmacology / Plants, Medicinal / Quality Control / Drugs, Chinese Herbal / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2006 Type: Article