Experimental research of Shuanghuang Shengbai granule on hematopoietic cells of leucopenia model mice / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 754-759, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-351763
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the leukogenic function of Shuanghuang Shengbai (SHSB) granule and the related mechanisms.</p><p><b>METHOD</b>Mouse leukopenic models were induced by radiation. Mice were divided into normal control group, model control group, positive control group-Li kejun tablet group and three different dose (high, middle, low-dose) groups of SHSB granule. The peripheral hemogram, thymus index (TI), spleen index (SI), bone marrow nucleated cell (BMNC) and colony forming unit-spleen (CFU-S) were evaluated. The proliferation of bone marrow cells was determined. The in vitro cultured colony forming unit granulocyte macrophage (CFU-GM) was estimated. The index of CD34+ cell in BMNC were determined by flow cytometry. The ultra-micro structure of bone marrow were observed by electromicroscope.</p><p><b>RESULT</b>(1)SHSB rranule could increase the WBC of model mice; (2)SHSB granule could increase BMNC and promote the proliferation of bone marrow cell; (3)SHSB granule could increase CFU-S, CFU-GM and CD34+ cell index in BMNC of model mice significantly; (4)SHSB Granule could also protect the bone marrow hemotopoietic microenvironment from the harm of radiation; (5)SHSB granule could increase the SI of model mice, indicating the enhancement of immunological function.</p><p><b>CONCLUSION</b>SHSB granule has apparent leukogenic function. The mechanism may be related to enhancing the proliferation of hematopoietic cells and protecting the bone marrow hemotopoietic microenvironment.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Plants, Medicinal
/
Bone Marrow Cells
/
Hematopoietic Stem Cells
/
Drugs, Chinese Herbal
/
Chemistry
/
Colony-Forming Units Assay
/
Antigens, CD34
/
Cell Proliferation
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
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