Determination of akebia saponin D in root of Dipacus asperoides by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 726-739, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-351773
ABSTRACT
<p><b>OBJECTIVE</b>To develop a quantitative method for the determination of akebia saponin D in root of Dipacus asperoides and provide scientific basis for quality control of D. asperoides.</p><p><b>METHOD</b>An IPLC analytical method was established using Kromasil ODS column with acetonitrile-water (3070) as mobile phase and the detection wavelength was 212 nm.</p><p><b>RESULT</b>The akebia saponin D in root extract were well separated by this method. The linear range is between 0.582 5-9.32 microg, r = 0.9999. The average recovery and RSD of repeatability are 100.3%, 2.3%, respectively.</p><p><b>CONCLUSION</b>This method is simple, accurate and reliable by evaluating the method validation data and can be used for the quality control of D. asperoides and its preparations.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Quality Control
/
Saponins
/
Chemistry
/
China
/
Chromatography, High Pressure Liquid
/
Ecosystem
/
Plant Roots
/
Dipsacaceae
Country/Region as subject:
Asia
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
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