HPLC determination of acteoside in Radix Rehmanniae / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 822-824, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-351783
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of acteoside in Radix Rehmanniae.</p><p><b>METHOD</b>The chromatographic conditions were as follows Polaris C18(4.6 mm x 250 mm, 5 microm) column, a mobile phase in gradient mode composed of acetonitrile 0.1% acetic acid solution, a flow rate of 1.0 mL x min(-1), and 334 nm as the detection wavelength.</p><p><b>RESULT</b>Acteoside showed good linear relationship at the range of 10-500 microg x mL(-1) (r = 0.9990). The average recovery was 100.1%, RS D 3.7%.</p><p><b>CONCLUSION</b>The proposed method promised to be applicable for the quality control of Radix Rehmanniae.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenols
/
Plants, Medicinal
/
Quality Control
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Plant Roots
/
Rehmannia
/
Glucosides
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
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