Studies on plantlet regeneration and propagation of Gentiana stramines / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 797-800, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-351792
ABSTRACT
<p><b>OBJECTIVE</b>To provide scientific basis for large scale production by studying the technique of tissue culture of Gentiana stramines.</p><p><b>METHOD</b>Callus was induced from germ-free stem segment of G. stramines on a MS medium supplemented with different hormones.</p><p><b>RESULT</b>The MS medium with 0.5-1.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the induction and proliferation of cluster bads. MS medium with 1.0 mg x L(-1) IAA and 3 mg x L(-1) BA was suitable for the induction of calli. MS medium with 1.0 mg x L(-1) IAA and 2.0-3.0 mg x L(-1) BA was suitable for the subculture of calli. MS medium with 2.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the differentiation of calli.</p><p><b>CONCLUSION</b>Aseptic seeding of G. stramines can be quickly propagated by shoot culture.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plant Growth Regulators
/
Plants, Medicinal
/
Regeneration
/
Plant Stems
/
Culture Media
/
Gentiana
/
Seedlings
/
Tissue Culture Techniques
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
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