Determination of saponin I in extracts of Allium macrostemnon / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 990-992, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-351803
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for determination of saponin I in bulb extracts of Allium macrostemon.</p><p><b>METHOD</b>The extract was refluxed by methanol and then filtrated. The filtrate was concentrated and the bioactive saponin was measured by HPLC-RID.</p><p><b>RESULT</b>The calibration curve was in good linearity over the range of 5-100 microg with the regression equation of Y= 9.35 x 10(4) X - 2.78 x 10(4) (r = 0.9998) and the average recovery was 101.1% (n = 5, RSD 1.6%).</p><p><b>CONCLUSION</b>The method is simple, quick and reproducible and can be applied in the quality control of A. macrostemon.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Quality Control
/
Saponins
/
Sterols
/
Allium
/
Plant Extracts
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Plant Roots
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
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