HPLC fingerprint of Rhizoma Polygoni Cuspidati / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the HPLC fingerprint of Rhizoma Polygoni Cuspidati (Polygonum cuspidatum).</p><p><b>METHOD</b>The HPLC separation was carried with Diamonsil C18 column and eluted with a gradient from methanol and 0.1% phosphoric acid, the detection wavelength was at 230 nm and recording 70 min. The similarity of chromatograms was compared by mean of the software from Zhongnan University.</p><p><b>RESULT</b>The constituents of P. cuspidatum were well separated by HPLC, and the similarity was above 0.80.</p><p><b>CONCLUSION</b>The method can be used for the study of fingerprints of Rhizoma Polygoni Cuspidati.</p>