Effect of transfection of p27(kipl) on lung cancer cells line A549 / 中国肺癌杂志

ABSTRACT

<p><b>BACKGROUND</b>To observe the suppressive effects of exogenous p27 gene on human lung cancer cell line A549.</p><p><b>METHODS</b>An adenovirus expression vector (pAd CMV-p27) containing 570 bp human full-length p27 cDNA was transfected into human lung cancer cell line A549. Expression of exogenous p27 gene was detected by dot-blot hybridization and laser co-focal system. MTT was adopted to measure the effects of exogenous p27 gene on cell cycle progression and cell features of the infected A549.</p><p><b>RESULTS</b>The mRNA and protein expression level of p27 was remarkably increased after transfecting with exogenous p27 gene. The apoptosis of infected A549 occurred and the progression of cell cycle was arrested in G1 phase.</p><p><b>CONCLUSIONS</b>p27(kipl) gene transfer may play a therapeutic role in the treatment of lung cancer.</p>