The mechanism of arsenic trioxide-inducing apoptosis of K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 558-562, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-352018
ABSTRACT
The aim was to investigate the mechanism of arsenic trioxide (ATO) inducing apoptosis of K562 cells that express P210Bcr-Abl. Apoptosis was analyzed by cell proliferation assay, morphological changes, DNA-PI staining and cell cycle analysis. ELISA kits were also used to analyze the concentration of cytosolic cytochrome C and activation of caspase-3. Transcriptional levels of Bcl-XL and Bcr-Abl were assayed by semiquantitative reverse transcription polymerase chain reaction (RT-PCR). The results showed that K562 cells were induced to apoptosis after exposure to 2.5 micromol/L ATO for at least 48 hours, and the cell cycle of K562 cells was arrested at the G2/M phase. Caspase-3 was activated and there was a cytosolic accumulation of cytochrome C. ATO could only reduce the transcriptional level of Bcl-XL, but could not down-regulate the Bcr-Abl transcriptional level. In conclusion, ATO can induce K562 cells to apoptosis. The signal pathway mediated by the cytosolic translocation of mitochondria cytochrome C is one of the mechanisms for ATO inducing apoptosis. And the decrease of Bcl-XL may induce more apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxides
/
Pharmacology
/
Arsenicals
/
Cell Cycle
/
Fusion Proteins, bcr-abl
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
K562 Cells
/
Caspases
/
Cytochromes c
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS