Genotyping of ABO loci in para-Bombay type individuals / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 513-516, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-352030
ABSTRACT
To study the molecular genetic basis of ABO alleles in para-Bombay type individuals, samples from five para-Bombay type individuals identified by serologic tests including absorption-elution tests, saliva neutralizing or inhibitor substances tests, were genotyped by using PCR-SSP based ABO genotyping. Exon 6 and exon 7 at the ABO locus for all 5 samples were sequenced. The results showed that the ABO genotypes of five para-Bombay samples were A102B1, A102B1, A102O1, A102B1, B1O1 respectively, the direct DNA sequencing results were in accordance with the results genotyped by PCR-SSP method, No novel nucleotide mutation was found at the exon 6 and exon 7 of ABO gene. In conclusion, the ABO genotyping assay by PCR-SSP provide a simple, rapid and accurate method for determining the ABO type of para-Bombay cases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
ABO Blood-Group System
/
Polymerase Chain Reaction
/
Chromosome Mapping
/
Sequence Analysis, DNA
/
Alleles
/
Genetics
/
Genotype
Type of study:
Prognostic study
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS