Detection of cytokine-secreting T lymphocytes in allogeneic reaction and a preliminary study on its clinical significance / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 498-502, 2004.
Article
in English
| WPRIM
| ID: wpr-352033
ABSTRACT
In order to explore a new way to study allogeneic reactive T lymphocytes, detection of cytokine-secreting T lymphocytes after allogeneic peripheral blood mononuclear cells (PBMNCs) stimulation and investigation of its clinical significance were performed. A novel cytokine secretion assay (CKSA) was first applied to detect T lymphocytes secreting cytokine including IFN-gamma, IL-4 and IL-10 at single cell level in human mixed lymphocytes reaction. IFN-gamma-secreting T cells from PBMNCs were then evaluated in 2 patients with acute graft versus host disease (aGVHD) after allogeneic bone marrow transplantation. The results showed that compared with IL-4 and IL-10 (which were 0.12 +/- 0.03% and 0.10 +/- 0.03% respectively), a sizable proportion of IFN-gamma-secreting T lymphocytes could be detected (1.12 +/- 0.13)% after allogeneic PBMNCs stimulation. Preliminary results indicated that frequency of IFN-gamma-secreting T lymphocytes correlated with the onset and severity of clinical aGVHD. In conclusion, it is feasible to detect IFN-gamma secreting T lymphocytes after allogeneic PBMNCs stimulation and to apply the CKSA technique for clinical identification of aGVHD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transplantation, Homologous
/
T-Lymphocytes
/
Acute Disease
/
Cytokines
/
Interleukin-4
/
Interferon-gamma
/
Interleukin-10
/
Bodily Secretions
/
Allergy and Immunology
/
Graft vs Host Disease
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Journal of Experimental Hematology
Year:
2004
Type:
Article
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