Preliminary establishment of an electrochemiluminescence immunoassay method for detection of influenza virus B / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 968-971, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-352131
ABSTRACT
To establish an electrochemiluminescence immunoassay (ECLIA) for the detection of influenza virus B (Flu B), we biotinylated the Flu B monoclonal antibody (mAb),labeled the paired Ab with ruthenium and used the streptavidin-coated microparticles in this study. The ECL intensity was linear with the concentrations of antigen of Flu B in the range of 0.55 microg/mL to 17.5 microg/mL, with a detection limit of 0.55 microg/mL (S/N= 3). The precision,sensitivity and specificity were evaluated. The established ECLIA for Flu B antigen in this study was specific, sensitive and precise. It may provide a convenient and rapid technique for clinical detection of Flu B.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Influenza B virus
/
Immunoassay
/
Luminescence
/
Electrochemical Techniques
/
Methods
/
Antibodies, Viral
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2013
Type:
Article
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