Investigation on bioactive protection of LEA protein for insulin by molecular simulation in the low-temperature drying process / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 854-859, 2013.
Article
in Zh
| WPRIM
| ID: wpr-352152
Responsible library:
WPRO
ABSTRACT
Nowadays various protein medicines are increasingly playing significant roles in the treatment of many diseases, but the bioactive structures of such kinds of protein medicines are unstable because they are heat sensitive. Therefore, it is very important to explore a protective method and to explain the protective mechanism of protein medicines. In the present research, insulin was chosen as a heat-sensitive protein medicine, and a Group 3 late embryogenesis abundant (LEA) protein was chosen as its bioactive protectant during desiccation. The results of replica exchange molecular dynamics simulation suggest that comparing with insulin without any protection, the bioactive 3D structure and secondary structure of the insulin protected by LEA protein were preserved very well. All analyzing results proved that the LEA protein was a good bioactive protectant for heat sensitive protein medicines.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Plant Proteins
/
Helminth Proteins
/
Chemistry
/
Protein Structure, Secondary
/
Cold Temperature
/
Drug Stability
/
Protein Interaction Domains and Motifs
/
Freeze Drying
/
Insulin
Limits:
Animals
Language:
Zh
Journal:
Journal of Biomedical Engineering
Year:
2013
Type:
Article