Separation and identification of down-regulated proteomics of intestinal mucosa in scalded rats / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 275-278, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-352271
ABSTRACT
<p><b>OBJECTIVE</b>To explore the pathogenesis of postburn intestinal mucosal injury in scalded rats.</p><p><b>METHODS</b>The rats inflicted with full thickness burn were employed as the model. The two-dimensional electrophoresis (2-DE) was employed to identify the down-regulating proteins from the differential proteins in scalded rats. Spot detection and matching were performed with Image Master 2D Elite. Mass spectrometry was performed on Bruker BIFLEX III TOF.</p><p><b>RESULTS</b>There are 34 points of proteins in intestinal mucosa in scalded rats which were down-regulated at 6 and 12 postburn hours. Among them 22 proteins were employed for the identification and analysis. Mitochondrial aconitase, alpha-propionyl -CoA carboxylase heavy chain A of F1-ATPase in rat liver, Troponin short-chain of hydroxyacyl-coenzyme A dehydrogenase, alpha-subunit of P-electronic transferring flavoprotein (ETF) were down-regulating proteins correlated with mitochondria in intestinal mucosa in severely scalded rats. Triosephosphate isomerase 1 and cytosolic epoxide hydrolase were down-regulating proteins participated in metabolism of scalded rats. Fibromodulin, dynein-like protein, Troponin-2 and myosin light chain 3 alkali (MLC) were down-regulating proteins correlated with cellular skeletal protein. Glucocorticoid-inducible protein, nuclear factor1-B2, BRCA1, transcriptive factor EB (estradiol benzoate), beta2 subunit of G-protein, N-methyl-D-aspartate receptor1 (NMDAR) were down-regulating proteins participated in postburn regulation of intestinal mucosa in scalded rats. T-cell receptor-V-delta 6 and Ig heavy chain V region protein 1 were down-regulating proteins correlated with the immunomodulation of intestinal mucosa in scalded rats.</p><p><b>CONCLUSION</b>The down-regulating proteins of intestinal mucosa in scalded rats exhibited close relationship with mitochondria.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Burns
/
Electrophoresis, Gel, Two-Dimensional
/
Down-Regulation
/
Cytokines
/
Rats, Sprague-Dawley
/
Proteome
/
Hormones
/
Intestinal Mucosa
/
Metabolism
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2003
Type:
Article
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