Anti-Mur screening and Mur antigen genotyping of voluntary blood donors in Guangzhou / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1833-1835, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-352323
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the frequency of anti-Mur and Mur antigen among blood donors in Guangzhou to provide evidence for guiding clinical transfusion and prenatal screening.</p><p><b>METHODS</b>DG Gel Coombs cards were used to screen active anti-Mur at 37 degrees celsius; from 2725 blood donors. Multiplex ligation-dependent probe amplification (MLPA) was used to genotype Mur antigen from 91 blood donors, and human anti-Mur serum was used to verify the phenotypes deduced from the genotypes.</p><p><b>RESULTS</b>The frequency of anti-Mur and genotyped Mur antigen was 0.04% (1/2725) and 6.59% (6/91), respectively, and the phenotyping results were consistent with the genotyping results.</p><p><b>CONCLUSION</b>The blood donors in Guangzhou show a low frequency of anti-Mur and a relatively high frequency of Mur antigen. Genotyping using MLPA allows Mur antigen genotyping when commercial anti-Mur is not available.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Blood Donors
/
Blood Group Antigens
/
China
/
Allergy and Immunology
/
Genotyping Techniques
/
Multiplex Polymerase Chain Reaction
/
Genetics
/
Genotype
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS