Establishment of a murine model of hepatic steatosis induced by chronic viral hepatitis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1722-1726, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-352347
ABSTRACT
<p><b>OBJECTIVE</b>To establish a animal model of hepatic steatosis induced by chronic viral hepatitis in C(57)BL/6 mice.</p><p><b>METHODS</b>C(57)BL/6 mice were randomly assigned to control group, high-fat diet group, mouse hepatitis virus strain A59 (MHV-A59) virus infection group, and high-fat diet plus virus infection group. At 13 weeks of the experiment, serum samples were collected to detect MHV antibodies and transaminase and lipid levels. The hepatic pathologies of the mice were examined with Oil red O staining of the frozen sections the and HE staining of paraffin-embedded sections.</p><p><b>RESULTS</b>The mice in the two virus infection groups showed strong positivity of MHV antibodies in the serum. Compared with the control group, the mice in high-fat diet group and the two virus infection groups had significantly increased AST and ALT levels with also elevated TC and LDL-C levels. The two virus infection groups both exhibited obvious pathologies in the liver characteristic of chronic viral hepatitis with increased lipid accumulation in the hepatocytes.</p><p><b>CONCLUSION</b>We have successfully established a mouse model of hepatic steatosis induced by chronic viral hepatitis, which provides the basis for further study of the disease mechanism.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Blood
/
Chronic Disease
/
Murine hepatitis virus
/
Disease Models, Animal
/
Fatty Liver
/
Diet, High-Fat
/
Hepatitis, Chronic
/
Mice, Inbred C57BL
/
Antibodies, Viral
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
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