Expressions of adiponectin and its receptors in the retina of normal and type 1 diabetic mice / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the expression of adiponectin and its receptors (AdipoRs) in the retina of normal mice and mice with type 1 diabetes mellitus (T1DM).</p><p><b>METHODS</b>C57BL/6 mice were randomly divided into control group and streptozotocin-induced T1DM group. Two months after the modeling, the total protein and adiponectin protein expression in the retina and choroid were measured using BCA method and enzyme-linked immunosorbent assay, respectively. Quantitative RT-PCR was performed to detect the mRNA expressions of AdipoRs in the retina and choroid, and Western blotting was employed to examine the protein expressions of AdipoRs in the retina.</p><p><b>RESULTS</b>Adiponectin and AdipoRs proteins were expressed in the retina and choroid in normal mice. The expressions of adiponectin and AdipoR1 were up-regulated in the retina of mice with T1DM while AdipoR2 expression exhibited no significant changes.</p><p><b>CONCLUSION</b>Adiponectin and AdipoR1 may play an important role in the evolvement of type 1 diabetic retinopathy.</p>