Molecular subtyping of Staphylococcus aureus isolated from a severe food-poisoning / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 672-676, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-352414
ABSTRACT
<p><b>OBJECTIVE</b>To study the molecular types of Staphylococcus aureus isolated from a severe food-poisoning and to trace the possible strains.</p><p><b>METHODS</b>Real-time PCR was applied to detect nuc gene as a specific marker for S. aureus, mecA gene encoding methicillin resistance and 5 other genes encoding staphylococcal enterotoxins (sea, seb, see, sed, see). Isolates were also performed with 16S rRNA oligonucleotide sequence analyzing by DNAStar MegAlign 5.0 software and pulse-field gel electrophoresis (PFGE) by BioNumerics Version 4.0 software.</p><p><b>RESULTS</b>The nuc gene was detected from the 10 isolated strains, sea and seb genes were detected from 7 strains. There were 4 16 S rRNA types and 5 PFGE types found from all the strains.</p><p><b>CONCLUSIONS</b>Three relative S. aureus strains were involved in the severe food-poisoning at least. Molecular subtyping might give a molecular epidemiological evidence and support the source tracing of an outbreak.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Staphylococcal Food Poisoning
/
Staphylococcus aureus
/
China
/
Epidemiology
/
Bacterial Typing Techniques
/
Classification
/
Electrophoresis, Gel, Pulsed-Field
/
Enterotoxins
/
Genetics
/
Microbiology
Limits:
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2008
Type:
Article
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