Identification and validation of novel C/EBPbeta-regulated genes in preadipocyte proliferation / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1190-1194, 2010.
Article
in English
| WPRIM
| ID: wpr-352594
ABSTRACT
<p><b>BACKGROUND</b>CCAAT/enhancer-binding protein beta (C/EBPbeta) is required for mitotic clonal expansion (MCE) during adipogenesis. It is still unclear how C/EBPbeta regulates MCE in the earlier differentiation programs of 3T3-L1 preadipocytes. The purpose of this paper was to understand why C/EBPbeta is required for preadipocyte proliferation, and identify new target genes of C/EBPbeta with chromatin immunoprecipitation (ChIP)-on-chip.</p><p><b>METHODS</b>Postconfluent growth-arrested 3T3-L1 preadipocytes were induced to differentiation using a standard differentiation protocol. ChIP was performed at 20 hours after induction with specific anti-C/EBPbeta antibodies. The precipitated DNA was amplified, labeled and hybridized with a mouse promoter microarray. Compared with the control in which the ChIP experiment was performed with non-specific antibody, only the genes with a signal increasing more than 2 fold were considered as candidate genes.</p><p><b>RESULTS</b>A total of 110 candidate genes were identified. BTG3 associated nuclear protein (SMAR1, Banp) and tripartite motif-containing 35 (Hls5, trim35) were two target genes among the 110 candidate genes which are involved in cell cycle regulation; the binding of C/EBPbeta to the promoter of banp and trim35 was verified by ChIP-PCR.</p><p><b>CONCLUSION</b>C/EBPbeta may regulate preadipocyte proliferation through activation of banp and trim35.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Protein Binding
/
Nuclear Proteins
/
Cell Differentiation
/
Polymerase Chain Reaction
/
Adipocytes
/
Cell Cycle Proteins
/
Oligonucleotide Array Sequence Analysis
/
CCAAT-Enhancer-Binding Protein-beta
/
Cell Biology
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2010
Type:
Article
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