Effect of repeated acute hypoxic treatment on the expression of alpha-synuclein in the mouse brain cortex / 生理学报
Sheng Li Xue Bao
; (6): 263-268, 2004.
Article
in Zh
| WPRIM
| ID: wpr-352782
Responsible library:
WPRO
ABSTRACT
An anti-alpha-synuclein (alpha-SYN) monoclonal antibody produced in our laboratory was used to investigate the effect of repeated acute hypoxic treatments on the expression of alpha-SYN in the mouse cerebral cortex. Western blot analysis showed that the expression levels of alpha-SYN in the cortex changed accordingly upon hypoxic exposure times, as that the alpha-synuclein level significantly increased after the first hypoxic exposure and then dropped down to the background level after the fourth hypoxic exposure. Immunohistochemical staining revealed that the alpha-SYN-immunopositive substance was localized not only in the nerve endings, but also within the nuclei of some neurons. The cell density of the neurons with alpha-SYN immunopositive nuclei was increased significantly after the first hypoxic exposure but returned back to control levels after the fourth hypoxic exposure. Our results indicate that both of the alpha-SYN expression level in the brain and the number of the neurons with alpha-SYN positive nuclei are affected by the repeated acute hypoxic treatments and that this modification is hypoxic time-dependent. The mechanism and the physiological significance underlying these changes need to be further investigated.
Full text:
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Index:
WPRIM
Main subject:
Phosphoproteins
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Brain
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Random Allocation
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Cerebral Cortex
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Brain Ischemia
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Ischemic Preconditioning
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Synucleins
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Alpha-Synuclein
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Genetics
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Metabolism
Limits:
Animals
Language:
Zh
Journal:
Sheng Li Xue Bao
Year:
2004
Type:
Article