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Effect of repeated acute hypoxic treatment on the expression of alpha-synuclein in the mouse brain cortex / 生理学报
Sheng Li Xue Bao ; (6): 263-268, 2004.
Article in Zh | WPRIM | ID: wpr-352782
Responsible library: WPRO
ABSTRACT
An anti-alpha-synuclein (alpha-SYN) monoclonal antibody produced in our laboratory was used to investigate the effect of repeated acute hypoxic treatments on the expression of alpha-SYN in the mouse cerebral cortex. Western blot analysis showed that the expression levels of alpha-SYN in the cortex changed accordingly upon hypoxic exposure times, as that the alpha-synuclein level significantly increased after the first hypoxic exposure and then dropped down to the background level after the fourth hypoxic exposure. Immunohistochemical staining revealed that the alpha-SYN-immunopositive substance was localized not only in the nerve endings, but also within the nuclei of some neurons. The cell density of the neurons with alpha-SYN immunopositive nuclei was increased significantly after the first hypoxic exposure but returned back to control levels after the fourth hypoxic exposure. Our results indicate that both of the alpha-SYN expression level in the brain and the number of the neurons with alpha-SYN positive nuclei are affected by the repeated acute hypoxic treatments and that this modification is hypoxic time-dependent. The mechanism and the physiological significance underlying these changes need to be further investigated.
Subject(s)
Full text: 1 Index: WPRIM Main subject: Phosphoproteins / Brain / Random Allocation / Cerebral Cortex / Brain Ischemia / Ischemic Preconditioning / Synucleins / Alpha-Synuclein / Genetics / Metabolism Limits: Animals Language: Zh Journal: Sheng Li Xue Bao Year: 2004 Type: Article
Full text: 1 Index: WPRIM Main subject: Phosphoproteins / Brain / Random Allocation / Cerebral Cortex / Brain Ischemia / Ischemic Preconditioning / Synucleins / Alpha-Synuclein / Genetics / Metabolism Limits: Animals Language: Zh Journal: Sheng Li Xue Bao Year: 2004 Type: Article