An improved method for acute isolation of neurons from the hippocampus of adult rats suitable for patch-clamping study / 生理学报
Acta Physiologica Sinica
;
(6): 112-117, 2004.
Article
in English
| WPRIM
| ID: wpr-352805
ABSTRACT
An improved method is described for fast and reliable isolation of neurons from hippocampus of adult rats by a combination of mechanical and enzymatic means. The procedure allows the isolation of neurons from 500-600-d-old rats (over 300 g), preserving the proximal dendritic structure without impairing the electrical characteristics of the cells. Morphologically distinct neurons can be recognized. Using cell-attached, inside-out and whole-cell configurations of patch clamp technique, it was shown that the enzymatically isolated neurons in hippocampus from rats weighing more than 300 g exhibited voltage-gated calcium, sodium and potassium currents, outwardly rectifying chloride channel and large conductance Ca(2+)-activated potassium channel currents. Approximately, 95% of healthy cells allowed the formation of giga-ohm seals.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Ion Channel Gating
/
Cell Separation
/
Rats, Wistar
/
Patch-Clamp Techniques
/
Cell Biology
/
Potassium Channels, Calcium-Activated
/
Hippocampus
/
Methods
/
Neurons
Limits:
Animals
Language:
English
Journal:
Acta Physiologica Sinica
Year:
2004
Type:
Article
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