Effect of LY294002 on adriamycin-induced epithelial-mesenchymal transition in human breast carcinoma cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 319-323, 2012.
Article
in English
| WPRIM
| ID: wpr-352908
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of LY294002 on the adriamycin- induced epithelial-mesenchymal transition in human breast carcinoma cells.</p><p><b>METHODS</b>Human breast carcinoma cells MCF-7 was cultured in vitro and then exposed to adriamycin with or without LY294002. The protein expression levels of Akt, phosphorylated-Akt (p-Akt), Snail, and E-cadherin was detected by Western blot analysis. The mRNA expressions of Snail and E-cadherin were determined by RT-PCR.</p><p><b>RESULTS</b>Adriamycin significantly increased the protein expression of Snail and depressed the protein expression of E-cadherin (P<0.05). The pre-treatment with LY294002 significantly reversed the changes of activities and levels of the above proteins (P<0.05).</p><p><b>CONCLUSION</b>LY294002 could reverse the adriamycin-induced epithelial-mesenchymal transition in human breast carcinoma cells by regulating the expressions of Snail and E-cadherin through suppressing PI3K/Akt signaling pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Transcription Factors
/
Breast Neoplasms
/
Signal Transduction
/
Doxorubicin
/
Cadherins
/
Morpholines
/
Chromones
/
Phosphatidylinositol 3-Kinases
Limits:
Humans
Language:
English
Journal:
Acta Academiae Medicinae Sinicae
Year:
2012
Type:
Article
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