Selection of culture media for the mass production of gamma delta T cells used in adoptive immunotherapy / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 644-648, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-352971
ABSTRACT
<p><b>OBJECTIVE</b>To select the optimal culture media for the mass production of gamma delta T cells used in adoptive immunotherapy.</p><p><b>METHODS</b>Three different culture media (RPMI-1640, AIM-V, and OpTmizer, with or without autologous serum) were used to culture gamma delta T cells. The survival rate, purity, proliferation efficiency, and biological functions of the expanded gamma delta T cells were examined and compared.</p><p><b>RESULTS</b>The survival rate of gamma delta T cells expanded in RPMI-1640 decreased over culture time. The purities of gamma delta T cells cultured in AIM-V or OpTmizer with or without serum were higher than those cultured in RPMI-1640. After two weeks of culture in the absence of serum, the purity and proliferation efficiency of gamma delta T cells cultured in OpTmizer were significantly higher than those cultured in RPMI-1640 (P < 0.05) and AIM-V (P < 0.05). gamma delta T cells in different culture media had similar CD107a expression and tumor necrosis factor-alpha production (P > 0.05). However, cells expanded in RPMI-1640 exhibited significantly weaker cytotoxicity against Daudi lymphoma cells than those expanded in OpTmizer (P < 0.05) and AIM-V (P < 0.05).</p><p><b>CONCLUSION</b>Due to low serum-dependence, high proliferation efficiency, and favorable biology function of expanded cells, OpTmizer is the most suitable medium for the mass production of gamma delta T cells used in adoptive immunotherapy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
T-Lymphocytes
/
Immunotherapy, Adoptive
/
Cell Culture Techniques
/
Culture Media
/
Cell Biology
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2011
Type:
Article
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