Separation and morphological observation of mouse testis seminiferous tubule epithelium / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To explore a simple and feasible technique to locate proteins during spermatogenesis.</p><p><b>METHODS</b>Various germ cells and somatic cells were separated by collagenase I and DNase I after the albuginea was removed. The cells were then smeared, dyed, and observed directly under fluorescence and confocal microscopy.</p><p><b>RESULTS</b>Germ cells at different steps were successfully identified by specific dyestuffs for acrosome and nucleolus.</p><p><b>CONCLUSION</b>A simple method for locating proteins during spermatogenesis was successfully developed.</p>