Screening and identification of natural antisense transcript in mouse cerebral cortex / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To screen and identify the possible existence of natural antisense transcript (NAT) within the mouse neocortex.</p><p><b>METHODS</b>Sixty-three cerebral cortex layer-specific genes were screened by bioinformatics prediction in mice, among which 31 mice with potential NATs were screened. NAT was identified using reverse transcription polymerase chain reaction (RT-PCR) and then cloned in pGEM-T Vector System for sequencing.</p><p><b>RESULTS</b>Among 31 genes predicted using bioinformatics, 8 were proved to be NAT positive by RT-PCR.</p><p><b>CONCLUSIONS</b>NATs exist in the mouse neocortex tissue during the development of cerebral cortex. NATs may influence mouse cortical development by regulating the related coding genes.</p>