Mapping of regulatory domain of T-protein from Escherichia coli / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To map the regulatory domain of Escherichia coli T-protein.</p><p><b>METHODS</b>Fragmentation cloning was employed in cloning of 11 fragments from T-protein. The regulatory activity of each fragment was determined respectively.</p><p><b>RESULTS</b>The regulatory domain of T-protein was located in the C-terminal 270 amino acids, which was the same location as PDH domain.</p><p><b>CONCLUSION</b>T-protein has no independent regulatory domain.</p>