Anti-inflammation and anti-oxidation effects of recombinant human superoxide dismutase on acute lung injury induced by meconium aspiration in infant rats / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 55-59, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-353249
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-inflammation and anti-oxidation effects of recombinant human CuZn superoxide dismutase(rhSOD) on acute lung injury (ALI) induced by meconium aspiration in rats.</p><p><b>METHODS</b>1 ml/kg of 20% human newborn meconium suspension was intratracheally (IT) administrated to induce the model of ALI in 32 male Sprage-Dawley rats, and the animals were then randomized to 4 groups 3 treatment groups with IT administration of 5, 10 and 20 mg/kg rhSOD dissolved in 1 ml/kg saline and the control group with IT administration of 1 ml/kg saline. The animals were killed after 24 h of treatments. The measurements included lung tissue wet/dry ratio, broncho-alveolar lavage fluid (BALF) protein, BALF protein/plasma protein (pulmonary permeability index, PPI),lung myeloperoxidase (MPO) and superoxide dismutase (SOD) activity, nitric oxide (NO) and 8-isoprostane levels. Lung injury score was also evaluated.</p><p><b>RESULTS</b>Compared with the control group, pulmonary MPO activity, NO and 8-isoprostane levels were significantly decreased and SOD activity was markedly increased in all rhSOD treatment groups (P<0.05 or 0.01). Compared with the rhSOD 5 mg/kg group, pulmonary 8-isoprostane level was further low in the rhSOD 20 mg/kg group(P=0.01). Lung injury score was decreased in rhSOD 20 mg/kg group (P<0.05). But there were no statistically differences in lung wet/dry, BALF protein and PPI among all groups.</p><p><b>CONCLUSION</b>The results suggest that a single IT dose of 5,10 or 20 mg/kg rhSOD can prevent lung damages in rats with ALI following meconium aspiration.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Superoxide Dismutase
/
Recombinant Proteins
/
Random Allocation
/
Anti-Inflammatory Agents, Non-Steroidal
/
Rats, Sprague-Dawley
/
Lung
/
Meconium
/
Antioxidants
Type of study:
Controlled clinical trial
/
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2005
Type:
Article
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