Establishment of two-dimensional electrophoresis of uterine leiomyoma for the proteome analysis / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 550-564, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-353262
ABSTRACT
<p><b>OBJECTIVE</b>To establish and optimize the two-dimensional electrophoresis (2-DE) of uterine leiomyoma for the proteome analysis.</p><p><b>METHODS</b>Run immobilized pH gradient (IPG)-isoelectric focusing electrophoresis as the first dimension, then vertical SDS-PAGE electrophoresis as the second dimension. A series of important steps,such as sample solubility, volume of loading, electrophoresis parameters and protocol for staining were optimized.</p><p><b>RESULTS</b>The 2-DE patterns of uterine leiomyoma and myometrium with good quality were obtained.</p><p><b>CONCLUSION</b>With optimal condition the two-dimensional electrophoresis of uterine leiomyoma can be obtained.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Uterine Neoplasms
/
Electrophoresis, Gel, Two-Dimensional
/
Chemistry
/
Proteome
/
Leiomyoma
/
Myometrium
/
Neoplasm Proteins
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS