Effect of HIV Tat protein on CCR5 expression in monocytes and infection with monocyte-tropic HIV strains / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 532-545, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-353266
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of HIV Tat protein on CCR5 expression of monocytes and HIV infection in monocytes.</p><p><b>METHODS</b>Membrane expression of CCR5 on monocytes was analyzed by flow cytometry. Stimulated with HIV Tat protein, monocytes were infected with monocyte-tropic HIV(Ba-L) and HIV gag p24 level in the supernatant was measured by ELISA methods.</p><p><b>RESULTS</b>HIV Tat protein increased CCR5 expression in human monocytes,which was inhibited by rabbit anti-Tat polyclonal antibody. Tat protein also increased p24 level after monocyte-tropic HIV-1(Ba-L) infected monocytes.</p><p><b>CONCLUSION</b>Tat increases CCR5 expression and HIV-1 infection in monocytes, which indicates that HIV Tat might be a key protein in HIV-1 infection.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Monocytes
/
Gene Products, tat
/
HIV Infections
/
HIV
/
Receptors, CCR5
/
Tat Gene Products, Human Immunodeficiency Virus
/
Flow Cytometry
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2004
Type:
Article
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