Study of an enhancer in promoter region of human colorectal cancer related ST13 gene / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 385-389, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-353298
ABSTRACT
<p><b>OBJECTIVE</b>To study the base sequence of an enhancer in up-stream 5'-flank near regulation region (from -595 to +74) of human colorectal cancer related gene ST13.</p><p><b>METHODS</b>Several deletion PCR primers were designed. Amplified DNA fragments of ST13 gene 5'-flank near region were cloned with pGEMT-EASY vector and sequenced; then subcloned into several pGL2 report vectors respectively. Equal quantitative recombined DNA was transfected into SW620 cell lines and the luciferase activity was checked.</p><p><b>RESULTS</b>Several amplified base sequence fragments (669 bp,263 bp,163 bp) in pGL2-Basic all enhanced and promoted luciferase gene expression strongly. The 47 bp and 101 bp fragments didn't promote luciferase gene expression. 101 bp fragment recombined with pGL2-Promoter enhanced luciferase gene expression distinctly (P<0.01), but the effect was less strong than the positive pGL2-Control(P<0.05).</p><p><b>CONCLUSION</b>The base sequence 101 bp (from -595 to -494) in up-stream 5'-flank near regulation region of colorectal cancer related gene ST13 is an enhancer regulating gene transcript.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription, Genetic
/
Molecular Sequence Data
/
Base Sequence
/
Transfection
/
Colorectal Neoplasms
/
Carrier Proteins
/
Enhancer Elements, Genetic
/
Promoter Regions, Genetic
/
Genes, Reporter
/
Tumor Suppressor Proteins
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2004
Type:
Article
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